High-density electrophysiological recordings in macaque using a chronically implanted 128-channel passive silicon
Liane Klein1,2,3,4, Frederick Pothof5,4, Bogdan C Raducanu6,7
1Ernst Strüngmann Institute (ESI) gGmbH for Neuroscience in Cooperation with Max Planck Society, Deutschordenstraße 46, D-60528, Frankfurt am Main, Germany.
Journal of Neural Engineering
|March 29, 2020
Summary
High-density neural probes using complementary metal-oxide-semiconductor (CMOS) technology enable stable, long-term recordings of brain activity in non-human primates. These probes offer high-quality local field potentials (LFPs) and multiunit activity (MUA) for over five months.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Materials Science
Background:
- Analyzing neural circuit interactions requires high-resolution, high-channel-count recordings.
- Complementary metal-oxide-semiconductor (CMOS) technology is essential for high-density neural probes.
- Validation of neural probes for non-human primate studies is critical.
Purpose of the Study:
- To validate neural probes for long-term recording in a head-fixed non-human primate model.
- To assess the recording capabilities of CMOS-based laminar microelectrode arrays.
- To prepare for future in-vivo studies in non-human primates.
Main Methods:
- Utilized passive CMOS silicon-based laminar probes with 128 electrodes (22.5 µm pitch).
- Implanted probes in the primary visual cortex (V1) of an awake, behaving macaque monkey.
- Employed a microdrive compatible with active CMOS probes for validation.
Main Results:
- Achieved stable local field potential (LFP) recordings for over five months.
- Recorded spiking multiunit activity (MUA) with sufficient quality for receptive field mapping throughout the recording period.
- Demonstrated successful probe insertion, readjustment, and stable chronic implantation.
Conclusions:
- Passive silicon CMOS probes facilitate high-quality, semi-chronic recordings of LFPs and MUAs exceeding five months.
- The developed microdrive allows for probe readjustment and reduces brain tissue movement.
- CMOS technology is suitable for high-density neural recordings in primate models.
