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Cellular Internalization of Beta-Carotene Loaded Polyelectrolyte Multilayer Capsules by Raman Mapping
Loredana F Leopold1, Oana Marișca2, Ioana Oprea1
1Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania.
Molecules (Basel, Switzerland)
|March 29, 2020
Summary
This study used Raman mapping to track beta-carotene capsules within human retinal cells. This technique enhances understanding of how these capsules improve stability and bioavailability for cellular uptake.
Area of Science:
- Biophysics
- Cell Biology
- Materials Science
Background:
- Raman mapping is a valuable technique for intracellular analysis of cells and molecules.
- Beta-carotene's stability and bioavailability are often limited, hindering its therapeutic potential.
Purpose of the Study:
- To encapsulate beta-carotene using a layer-by-layer method to improve its properties.
- To utilize Raman mapping to characterize these beta-carotene capsules.
- To monitor the cellular uptake of these capsules by human retinal epithelial cells.
Main Methods:
- Layer-by-layer encapsulation of beta-carotene.
- Characterization of encapsulated beta-carotene using Raman mapping.
- Monitoring cellular uptake in D407 cells via Raman mapping.
Main Results:
- Successfully mapped the distribution of beta-carotene within the capsules.
- Localized the capsules within the D407 cells.
- Differentiated between the capsules and other cellular components using Raman spectroscopy.
Conclusions:
- Raman mapping is effective for characterizing beta-carotene capsules.
- The layer-by-layer technique successfully enhanced beta-carotene stability and bioavailability.
- Raman mapping can track intracellular capsule localization and differentiate them from cellular structures.
