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Gene structure and in situ transcript localization of pathogenesis-related protein 1 in parsley
I E Somssich1, E Schmelzer, P Kawalleck
1Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Federal Republic of Germany.
Abstract:
We have analysed three nearly full-length cDNAs complementary to mRNAs encoding two PR1 (pathogenesis-related, class 1) proteins in parsley (Petroselinum crispum). Furthermore, one selected genomic clone containing the PcPR1-1 gene was investigated in detail. The structural organization and possible regulatory elements in the 5' flanking region of this gene are presented. In situ RNA hybridization in fungus-infected parsley leaf tissue demonstrated rapid and massive PR1 mRNA accumulation around infection sites.