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Updated: Dec 25, 2025

The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability
Published on: April 28, 2022
Iron application improves yield, economic returns and grain-Fe concentration of mungbean
Abdul Majeed1, Waqas Ahmed Minhas1, Noman Mehboob1
1Department of Agronomy, Bahauddin Zakariya University, Multan, Pakistan.
Abstract:
Malnutrition is among the biggest threats being faced globally, and Pakistan is among the countries having high malnutrition rate. Pulses grown in Pakistan have lower amounts of micronutrients, especially iron (Fe) in grains compared to developed world. Biofortification, -a process of integrating nutrients into food crops-, provides a sustainable and economic way of increasing minerals/micronutrients' concentration in staple crops. Mungbean fulfills protein needs of large portion of Pakistani population; however, low Fe concentration in grains do not provide sufficient Fe. Therefore, current study was conducted to infer the impact of different Fe levels and application methods on yield, economic returns and grain-Fe concentration of mungbean. Mungbean was sown under four levels of Fe, i.e., 0, 5, 10 and 15 kg Fe ha-1 applied by three methods, i) as basal application (whole at sowing), ii) side dressing (whole at 1st irrigation) and iii) 50% as basal application + 50% side dressing (regarded as split application). Iron levels and application methods significantly influenced the allometry, yield, economic returns and grain-Fe concentration of mungbean. Split application of 15 kg Fe ha-1 had the highest yield, economic returns and grain-Fe concentration compared to the rest of Fe levels and application methods. Moreover, split application of 15 kg Fe ha-1 proved a quick method to improve the grain-Fe concentration and bioavailability, which will ultimately solve the Fe malnutrition problem of mungbean-consuming population in Pakistan. In conclusion, split application of Fe at 15 kg ha-1 seemed a viable technique to enhance yield, economic returns, grain-Fe concentration and bioavailability of mungbean.
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