Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transmission Electron Microscopy01:15

Transmission Electron Microscopy

6.7K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
6.7K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

12.7K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
12.7K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

14.6K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
14.6K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.7K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.7K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

5.1K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
5.1K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.1K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemical and sensory profiling of fermented, washed, and artificially flavored coffee beans: Insights into flavour quality, authenticity, and food safety implications.

Food chemistry·2026
Same author

Unlocking Zn-Ion Diffusion in Disordered Rocksalt Cathodes for Nonaqueous Zn-Ion Batteries.

Angewandte Chemie (International ed. in English)·2026
Same author

Nanoengineering Interfacial Reconstruction in Cu<sub>2</sub>O@SiO<sub>2</sub> Catalysts to Tune C-C Coupling and Deep Hydrogenation in CO<sub>2</sub> Electroreduction.

ACS applied materials & interfaces·2026
Same author

Design, synthesis, biological evaluation, DFT and molecular docking studies of novel isoxazolines containing cyclic amide.

Bioorganic chemistry·2026
Same author

Air-permeable hydrogels through viscoelastic phase separation of aerogels.

Nature·2026
Same author

Post-stroke acute heart failure in patients with large vessel occlusion undergoing endovascular treatment: A pooled analysis of individual patient data from multicenter studies with mediation analysis.

PLoS medicine·2026

Related Experiment Video

Updated: Dec 25, 2025

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
07:37

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

Published on: December 20, 2012

13.2K

Liquid cell transmission electron microscopy and its applications.

Shengda Pu1, Chen Gong1, Alex W Robertson1

  • 1Department of Materials, University of Oxford, Parks Road, Oxford OX1 3PH, UK.

Royal Society Open Science
|March 29, 2020
PubMed
Summary

Transmission electron microscopy (TEM) now allows direct nanoscale observation of liquid dynamics using microchip liquid cells. This in situ TEM technique provides unique insights into nanomaterial synthesis, battery science, and biological processes.

Keywords:
in situ transmission electron microscopyliquid cell transmission electron microscopytransmission electron microscopy

More Related Videos

Electron Cryotomography of Bacterial Cells
14:23

Electron Cryotomography of Bacterial Cells

Published on: May 6, 2010

26.1K
Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
09:53

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

Published on: September 13, 2021

7.4K

Related Experiment Videos

Last Updated: Dec 25, 2025

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
07:37

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

Published on: December 20, 2012

13.2K
Electron Cryotomography of Bacterial Cells
14:23

Electron Cryotomography of Bacterial Cells

Published on: May 6, 2010

26.1K
Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
09:53

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

Published on: September 13, 2021

7.4K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Transmission electron microscopy (TEM) is crucial for materials structure analysis.
  • Recent advancements include aberration correction, cryogenic TEM, and in situ dynamic system observation.
  • Silicon-chip architectures enable versatile experiments within the TEM.

Purpose of the Study:

  • To review in situ TEM experiments for observing liquid dynamics at the nanoscale.
  • To focus on microchip-encapsulated liquid cell TEM.
  • To discuss the technique's strengths, weaknesses, and applications.

Main Methods:

  • Utilizing silicon-chip architecture for in situ experiments under high vacuum.
  • Employing microchip-encapsulated liquid cells to safely enclose fluids within the TEM.
  • Performing in situ imaging of liquid phase reactions.

Main Results:

  • Demonstrated safe enclosure of fluids for nanoscale liquid dynamics observation.
  • Provided unique insights into nanomaterial synthesis and manipulation.
  • Offered new perspectives in battery science and biological cell studies.

Conclusions:

  • In situ liquid cell TEM is a powerful technique for fundamental process understanding.
  • The method enhances insights across diverse scientific fields.
  • Addressing current challenges will further expand the technique's capabilities.