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Updated: Dec 25, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
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miR-335 Acts as a Tumor Suppressor and Enhances Ionizing Radiation-Induced Tumor Regression by Targeting ROCK1

Yanfeng Cheng1, Peng Shen1

  • 1Department of Dermatology, Shengjing Hospital of China Medical University, Shenyang, China.

Frontiers in Oncology
|March 29, 2020
PubMed

Insights

MicroRNA-335 (miR-335) may overcome melanoma radioresistance. Lower miR-335 levels correlate with advanced melanoma, and its restoration can enhance radiotherapy effectiveness against skin cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Melanoma, a skin cancer, exhibits poor outcomes with advanced or recurrent disease, often showing resistance to radiotherapy.
  • Current integrative therapies for melanoma have limitations, necessitating research into radioresistance mechanisms.

Purpose of the Study:

  • To investigate the role of microRNAs (miRNAs) in melanoma radioresistance.
  • To identify specific miRNAs involved in recurrent melanoma post-radiotherapy and elucidate their therapeutic potential.

Main Methods:

  • Differential expression analysis of miRNAs in melanoma tissues.
  • Bioinformatic prediction and experimental validation of miRNA targets.
  • In vitro assays assessing cell proliferation, migration, and radioresistance.
  • In vivo studies using mouse models to evaluate tumor regression.

Main Results:

  • miR-335 expression was significantly decreased in melanoma tissues and correlated negatively with advanced melanoma stages.
  • miR-335 directly targets ROCK1, inhibiting melanoma cell proliferation, migration, and radioresistance.
  • Overexpression of miR-335 enhanced X-ray-induced tumor regression in a mouse model.

Conclusions:

  • miR-335 plays a crucial role in modulating melanoma radiosensitivity by targeting ROCK1.
  • Restoring miR-335 levels presents a potential therapeutic strategy to improve radiotherapy outcomes for melanoma patients.
Keywords:
ROCK1apoptosisionizing radiationmelanomamiR-335resistance

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