Liquid Chromatography Tandem Mass Spectrometry Analysis of Proteins Associated with Age-Related Disorders in Human Pituitary Tissue
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Updated: Dec 25, 2025
Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
Published on: April 2, 2018
Paul C Guest1, Daniel Martins-de-Souza2,3,4
1Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil. paulcguest@yahoo.com.
Abstract:
The pituitary gland is involved in multiple physiological functions, including growth, development, reproduction, stress adaptation, osmotic balance, body composition, skin pigmentation, and aging. Advancing age is characterized by dysfunctions in multiple physiological signaling mechanisms, concomitant with perturbed patterns of pituitary hormone release as well as disrupted rhythmic secretion of virtually all pituitary hormones. This chapter presents a liquid chromatography tandem mass spectrometry (LC-MS/MS) protocol for analysis of the protein content in post-mortem pituitary glands. It was of special interest to determine if proteins previously linked with aging and age-related disorders could be identified to support further studies in this field using proteomic profiling approaches. This included hormones, hormone-processing enzymes, histones, oxidation-reduction enzymes, and others.
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