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Histomorphometric Analysis of Anti-Aging Properties on Rat Skin
Idha Kusumawati1, Kresma Oky Kurniawan2, Subhan Rullyansyah2
1Pharmacognosy and Phytochemistry Department, Faculty of Pharmacy, Airlangga University, Surabaya, East Java, Indonesia. idha-k@ff.unair.ac.id.
Abstract:
We present a histomorphometric analysis to enable quantitative measurement of anti-aging activity of topical substances in rat skin. In this method, the measured parameters were closely related to changes that occur on the skin caused by exposure to UV light. We used this protocol to determine the anti-aging activities of an ethanolic extract of Curcuma heyneana rhizome and retinoic acid by a measurement of the changes in epidermal thickness, the number of sunburned cells, the number of fibroblasts, and the space between collagen fibers.

