Multiplex Analysis of Circulating Hormone Levels in Rat Models of Age-Related Diseases

This chapter describes the application of multiplex immunoassay of hormones which are known to be present at different levels during aging and may therefore be precipitous in age-related diseases. As an example, we have analyzed serum from 12-week-old rats using multiplexes for the hormones insulin, leptin, growth hormone, corticosterone, and testosterone, which have been implicated in sarcopenia. The same technique can be used in the investigation of other sarcopenia biomarkers as well as in other disease cases involving both clinical and preclinical biomarker studies.