Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Dec 25, 2025

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs
06:58

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Published on: May 18, 2022

1.4K

Disruption in murine Eml1 perturbs retinal lamination during early development.

G B Collin1, J Won1, M P Krebs1

  • 1The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine, 04609, USA.

Scientific Reports
|March 30, 2020
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Time-resolved Rayleigh scattering measurements of methane clusters for laser-cluster fusion experiments.

PloS one·2021
Same author

Impact of stringent response in proteinuria on long-term renal outcomes in proliferative lupus nephritis.

Lupus·2019
Same author

Creating virtual 3-dimensional models for teaching pre-clinical tooth preparation: Students' usages and perceptions.

European journal of dental education : official journal of the Association for Dental Education in Europe·2018
Same author

TRPM7 Mediates Mechanosensitivity in Adult Rat Odontoblasts.

Journal of dental research·2018
Same author

Piezo2 Expression in Mechanosensitive Dental Primary Afferent Neurons.

Journal of dental research·2017
Same author

A DN-mda5 transgenic zebrafish model demonstrates that Mda5 plays an important role in snakehead rhabdovirus resistance.

Developmental and comparative immunology·2015

The Eml1 gene is crucial for proper neural development and cell positioning in the mammalian brain and retina. Disruption leads to severe lamination defects, impacting neuronal function and tissue architecture.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • Proper neuronal migration and positioning (lamination) are essential for functional neural networks in the central nervous system.
  • The molecular mechanisms governing retinal and cerebrocortical lamination are not fully understood.
  • Defects in lamination can result in mispositioned neurons, disrupted connections, and abnormal function.

Purpose of the Study:

  • To investigate the role of the Eml1 gene in mammalian neural development, specifically focusing on retinal lamination.
  • To characterize the phenotype of a novel murine model (tvrm360) with a mutation in the Eml1 gene.

Main Methods:

  • Generation and analysis of a novel murine model (tvrm360) with a nonsense mutation in the Eml1 gene.
  • Histological examination of retinal and cerebrocortical tissues during development.

More Related Videos

Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium
16:47

Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium

Published on: February 27, 2011

50.9K
Manipulating the Murine Lacrimal Gland
05:56

Manipulating the Murine Lacrimal Gland

Published on: November 18, 2014

21.5K

Related Experiment Videos

Last Updated: Dec 25, 2025

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs
06:58

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Published on: May 18, 2022

1.4K
Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium
16:47

Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium

Published on: February 27, 2011

50.9K
Manipulating the Murine Lacrimal Gland
05:56

Manipulating the Murine Lacrimal Gland

Published on: November 18, 2014

21.5K
  • Assessment of neuronal positioning, cell morphology, and differentiation.

    • Main Results:

    • The Eml1 mutation in tvrm360 mice caused subcortical heterotopia, hydrocephalus, and retinal disorganization.
    • Eml1 disruption led to abnormal positioning of photoreceptor cell nuclei and Müller cell bodies in the retina.
    • While ectopic photoreceptors formed cilia and synapses, they failed to develop outer segments, indicating a late differentiation defect.
    • Mitotic nuclei in the retinal neuroepithelium showed basal displacement, suggesting an early defect in progenitor cell positioning.

    Conclusions:

    • EML1 plays a critical role in early eye development, particularly in retinal progenitor cell positioning and proliferation.
    • Proper retinal lamination during development is dependent on EML1 function.
    • EML1 is essential for establishing normal retinal architecture and neuronal positioning, with implications for understanding neurodevelopmental disorders.