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Field Isolation and Cultivation of Trypanosomatids from Insects

Julius Lukeš1,2, Jan Votýpka3,4

  • 1Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic. jula@paru.cas.cz.

Methods in Molecular Biology (Clifton, N.J.)
|March 30, 2020
PubMed

Keywords:
AxenizationCultivationFieldInsectsIsolationTrypanosomaTrypanosomatids

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American Trypanosomiasis01:22

American Trypanosomiasis

Chagas disease, or American trypanosomiasis, is a vector-borne parasitic infection caused by Trypanosoma cruzi, a flagellated protozoan (kinetoplastid) of the family Trypanosomatidae. The disease is endemic in Latin America, although cases are increasingly reported worldwide due to human migration. Transmission most commonly occurs when feces of infected triatomine bugs contaminate bite wounds or mucosal surfaces; additional routes include congenital, transfusional, transplant-related, and oral...