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Updated: Dec 25, 2025

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
Published on: August 19, 2014
Light Microscopy in Trypanosomes: Use of Fluorescent Proteins and Tags
1Warwick Medical School, University of Warwick, Coventry, UK. Samuel.Dean@warwick.ac.uk.
Abstract:
Fluorescence microscopy enables the localization of proteins to specific structures within a cell which have either been fused to a fluorescence protein or detected by immunofluorescence. Here, we describe the various procedures that can be used to prepare both the procyclic form and bloodstream form of the human pathogen Trypanosoma brucei for fluorescence microscopy. The choice of procedure to be used is determined by various parameters, including protein characteristics and the scientific question being investigated.
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