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Updated: Dec 25, 2025

Polysome Profiling in Leishmania, Human Cells and Mouse Testis
Published on: April 8, 2018
2D Gel Electrophoresis Analysis of Leishmania Proteomes
Christina Naula1, Richard Burchmore2
1Institute of Infection, Immunity and Inflammation and Glasgow Polyomics, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Abstract:
2D gel electrophoresis enables resolution of intact proteins in complex mixtures and is thus useful for comparative proteomic analysis, particularly of posttranslationally modified proteoforms that might not be distinguished by shotgun proteomic analysis of peptides. 2D gel electrophoresis is a multistep procedure that can require sample-specific optimization. We present a comprehensive protocol that is effective for 2D electrophoretic analysis of proteins from Leishmania promastigotes and may also be employed for Leishmania amastigotes and for trypanosomes.
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