Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Molecular and clinical characteristics and prognosis of myelodysplastic neoplasms with biallelic TET2 inactivation].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2026
Same author

[Pathophysiology-based phenotyping and clinical characterization of obesity].

Zhonghua nei ke za zhi·2026
Same author

[Clinical characteristics and prognosis of myelodysplastic neoplasms with chromosome 1 abnormalities].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2026
Same author

[Myelodysplastic neoplasms with acute myeloid leukemia-like mutations: clinical features, molecular profiles, and prognosis].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same author

[Differences in clinical and laboratory features and survival between Chinese and Western patients with myelodysplastic neoplasm].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same author

Associations of socioeconomic status and malnutrition with survival in adults with cancer in the UK Biobank: A prospective cohort study.

Public health·2025
Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026
Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026
Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026
Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026
Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026
Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026
See all related articles

Related Experiment Video

Updated: Dec 25, 2025

Author Spotlight: Advancing Organoid Generation for Drug Development Using iPSCs
06:38

Author Spotlight: Advancing Organoid Generation for Drug Development Using iPSCs

Published on: March 15, 2024

2.0K

Cell-derived matrices (CDM)-Methods, challenges and applications.

H W Cheng1, M T Yuan1, C W Li1

  • 1Tissue Engineering Laboratory, Biomedical Engineering Programme, Department of Mechanical Engineering, The University of Hong Kong, Pokfulam, Hong Kong.

Methods in Cell Biology
|March 31, 2020
PubMed
Summary
This summary is machine-generated.

This study reviews methods for recreating the extracellular matrix (ECM) niche, focusing on cell-derived matrix (CDM) from various cell types. It highlights approaches, applications, and future directions for engineering cellular microenvironments.

Keywords:
Cell-derived matrixChondrocytesChondrogenesisDecellularizationExtracellular matrixMesenchymal stem cellsMicroencapsulationNucleus pulposus cellsOsteogenesis

More Related Videos

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

7.9K
A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

17.2K

Related Experiment Videos

Last Updated: Dec 25, 2025

Author Spotlight: Advancing Organoid Generation for Drug Development Using iPSCs
06:38

Author Spotlight: Advancing Organoid Generation for Drug Development Using iPSCs

Published on: March 15, 2024

2.0K
Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

7.9K
A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

17.2K

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • The extracellular matrix (ECM) is crucial for cellular support and signaling within the microenvironment.
  • Engineering the ECM niche is vital for studying and controlling cellular behavior.
  • Current methods for ECM reconstitution range from simple coatings to complex tissue decellularization.

Purpose of the Study:

  • To provide an overview of ECM niche reconstitution strategies.
  • To focus on cell-derived matrix (CDM) generation from diverse cell sources.
  • To discuss applications, challenges, and future perspectives of CDM.

Main Methods:

  • Review of various ECM reconstitution approaches, including ECM molecule coating and native tissue decellularization.
  • Detailed examination of cell-derived matrix (CDM) generation methods.
  • Analysis of CDM derived from mature cells (chondrocytes, nucleus pulposus cells), undifferentiated stem cells (mesenchymal stem cells - MSCs), and differentiating MSCs in 2D and 3D models.

Main Results:

  • Successful reconstitution of complex ECM niches using CDM from various cell types.
  • Identification of key factors for successful CDM applications.
  • Elucidation of challenges associated with CDM generation and utilization.

Conclusions:

  • Cell-derived matrix (CDM) offers a versatile approach to reconstitute complex ECM niches.
  • Careful consideration of application-specific factors and challenges is necessary for effective CDM use.
  • Future perspectives suggest significant potential for CDM in regenerative medicine and cell-based studies.