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Related Experiment Videos

Methods in cell biology: Cell-derived matrices.

Elizabeth Pavez Loriè1, Petra Boukamp2

  • 1Leibniz Institute of Environmental Medicine, Düsseldorf, Germany.

Methods in Cell Biology
|March 31, 2020
PubMed
Summary

This study presents a new fibroblast-derived matrix-based model for long-term three-dimensional (3D) skin equivalents. This advanced model overcomes limitations of previous scaffolds, improving skin research and skin cancer modeling.

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Area of Science:

  • Tissue Engineering
  • Dermatology
  • In Vitro Modeling

Background:

  • Three-dimensional (3D) in vitro skin models are crucial for skin research.
  • Early models used collagen-based dermal equivalents (DEs), facing limitations like restricted survival time and premature fibroblast activation.
  • Subsequent scaffold-based DEs improved longevity but suffered from opaqueness and non-specific tumor invasion patterns.

Purpose of the Study:

  • To present a protocol for a fibroblast-derived matrix-based model for 3D skin equivalents.
  • To overcome the limitations of previous skin models, including opaqueness and artifactual invasion patterns.
  • To provide a basis for future advancements in skin research and skin cancer modeling.

Main Methods:

  • Development of a scaffold-based dermal equivalent (DE) using fibrin, allowing fibroblast-mediated matrix reorganization.
Keywords:
3D modelsCell-derived matrixDermal equivalentECMFibroblastHuman modelKeratinocyteLong-termModeling approachOrganProtocolSelf-assembledSkin equivalentsTissue

Related Experiment Videos

  • Co-culture of the DE with normal human skin keratinocytes to form skin equivalents.
  • Implementation of a fibroblast-derived matrix-based model, building upon prior work.

    • Main Results:

    • Achieved long-term skin equivalents with successful cultivation for several months.
    • Circumvented premature fibroblast activation and tissue destruction common in earlier models.
    • Avoided opaqueness and non-specific invasion patterns observed in scaffold-based models.

    Conclusions:

    • The fibroblast-derived matrix-based model offers a significant improvement over previous 3D skin models.
    • This model provides a more authentic and transparent tissue for advanced skin research.
    • It serves as a foundational tool for future studies in skin biology and cancer.