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A TaqMan-based quantitative real-time PCR assay for identification of the goose circovirus

Kan-Kan Yang¹, Dong-Dong Yin¹, Liang Xu¹

¹Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, Anhui Agricultural University, Shushan District West Yangtze River Road 130#, Hefei, 230036, Anhui Province, People's Republic of China.

Molecular and Cellular Probes

|March 31, 2020

Abstract:

Goose circovirus (GoCV) is a potential immunosuppressive virus that poses a great hazard to the goose industry and has been shown to be widely distributed throughout China. We have established a fast, sensitive and highly specific TaqMan real-time quantitative PCR detection method for this virus. Specific primers and probes were designed against the conserved regions of the genomic GoCV Rep gene. The results showed that the assay was highly specific and sensitive for GoCV and did not cross-react with other non-targeted waterfowl viruses. The established method will be helpful for epidemiological detection and may be effective in the prevention and control of the disease.

Keywords:

Goose circovirus Real-time PCR Rep gene TaqMan

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Real Time RT-PCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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