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Functionalized Pt(II) and Ir(III) NIR Emitters and Their Covalent Conjugates with Polymer-Based Nanocarriers
Ilya S Kritchenkov1, Daniil D Zhukovsky1, Abdelrahman Mohamed1,2
1Institute of Chemistry, Saint-Petersburg State University, Saint-Petersburg 198504, Russia.
Bioconjugate Chemistry
|April 1, 2020
Summary
Novel luminescent platinum and iridium nanoparticles were developed for effective cell penetration. Surface charge modification influences cellular uptake, with positively charged microparticles and heparin-coated nanoparticles showing enhanced cellular entry for potential drug delivery applications.
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Development of novel luminescent materials for biomedical applications.
- Need for targeted drug delivery systems with efficient cellular uptake.
- Challenges in controlling nanoparticle surface properties for biological interactions.
Purpose of the Study:
- Synthesize and characterize NIR-emitting platinum and iridium complexes.
- Fabricate core-shell luminescent micro- and nanoparticles for drug delivery.
- Investigate the effect of surface charge modification on cellular penetration.
Main Methods:
- Synthesis and spectroscopic characterization of platinum and iridium complexes.
- Preparation of biodegradable polymer core-shell nanoparticles.
- Surface functionalization with poly(l-lysine) and heparin.
- Zeta-potential measurements and dynamic laser scanning for size analysis.
- Cellular uptake studies in cancer cells, stem cells, and fibroblasts.
Main Results:
- Successfully synthesized NIR-emitting Pt and Ir complexes with reactive succinimide groups.
- Created stable core-shell micro- and nanoparticles with tunable sizes (720-1480 nm for microparticles, 210-230 nm for nanoparticles).
- Demonstrated effective cellular penetration of all particle types into various cell lines.
- Nanoparticles showed higher penetration than microparticles.
- Positively charged microparticles exhibited greater cellular uptake than negatively charged ones.
- Heparin-coated nanoparticles demonstrated improved cellular entry compared to poly(l-lysine) coated ones.
Conclusions:
- Developed versatile luminescent nanoparticles with potential for drug delivery.
- Surface charge plays a critical role in cellular uptake efficiency.
- Optimized surface modifications can enhance nanoparticle interaction with cells.
