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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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3-Hydroxyhexanoate-based polycationic nanoparticle system for delivering reprogramming factors.

Hanife Sevgi Varlı1, Funda Alkan1, Fatma Ceren Kırmızıtaş1

  • 1Molecular Biology and Genetic Department, Yıldız Technical University, Istanbul, Turkey.

Journal of Microencapsulation
|April 1, 2020
PubMed
Summary

Researchers developed polycationic nanoparticles (PXNs) for delivering reprogramming factors to fibroblast cells. These PXNs show significant transfection efficiency, offering a promising tool for cell reprogramming applications.

Keywords:
PXNReprogramming factorsfibroblastnanoparticlenon-viral

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Area of Science:

  • Biotechnology
  • Materials Science
  • Cell Biology

Background:

  • Non-viral carriers are crucial for gene delivery.
  • Developing effective carriers for reprogramming factors is essential for regenerative medicine.

Purpose of the Study:

  • To develop a polycationic non-viral carrier for delivering reprogramming factors.
  • To evaluate the efficacy and safety of the developed carrier for L929 fibroblast cell transfection.

Main Methods:

  • Preparation of polycationic nanoparticles (PXNs) using PHBHHx and the solvent diffusion method.
  • Assessment of cytotoxicity via MTT assay.
  • Evaluation of transfection efficiency using GFP expression and RT-qPCR for reprogramming factors (Oct4, Klf4, Sox2).

Main Results:

  • PXNs exhibited a zeta potential of 32.9 ± 0.41 mV and a size of 177.6 ± 0.80 nm.
  • Cell viability ranged from 91.8% to 42.1%, indicating dose-dependent effects.
  • Transfection efficiency reached 71.6%, with significantly increased reprogramming factor expression after 11 cycles.

Conclusions:

  • The newly developed PXN is an effective non-viral carrier for delivering reprogramming factors.
  • PXNs demonstrate potential for use in cell reprogramming applications.
  • Further research can explore PXNs for broader gene delivery applications.