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β-Glucan augments IL-1β production by activating the JAK2/STAT3 pathway in cultured rabbit keratinocytes

Wudian Xiao1, Lu Fu2, Congwei Gu2

  • 1Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China; Laboratory Animal Center, Southwest Medical University, Luzhou, 646000, China.

Microbial Pathogenesis
|April 1, 2020
PubMed

Insights

Beta-glucan, a key component of Trichophyton mentagrophytes, triggers inflammatory responses in rabbit skin keratinocytes. This study reveals beta-glucan

Area of Science:

  • Veterinary Dermatology
  • Immunology
  • Mycology

Background:

  • Trichophyton mentagrophytes (T. mentagrophytes) is a primary cause of dermatophytosis in rabbits.
  • Beta-glucan is a major pathogen-associated molecular pattern of T. mentagrophytes, but its role in rabbit skin infections is not well understood.
  • Keratinocytes (KC) are crucial skin cells and the initial defense against fungal invasion.

Purpose of the Study:

  • To investigate the effects of beta-glucan on rabbit keratinocytes (KC).
  • To elucidate the role of the JAK2/STAT3 signaling pathway in beta-glucan-induced immune responses in rabbit KC.

Main Methods:

  • Rabbit KC were treated with beta-glucan at varying concentrations.
  • Cell proliferation was assessed, and optimal beta-glucan concentration (20 ug/ml) was determined.
  • Secretions of CXCL8, CXCL11, and IL-1β were measured.
  • Expressions of JAK2 mRNA, STAT3 mRNA, and p-STAT3 protein were analyzed.
  • JAK2 silencing and overexpression experiments were conducted to study pathway involvement.

Main Results:

  • Beta-glucan (20 ug/ml) inhibited KC proliferation by 10% and significantly increased CXCL8, CXCL11, and IL-1β secretions.
  • Beta-glucan induced the expression of JAK2 mRNA, STAT3 mRNA, and p-STAT3 protein.
  • JAK2/STAT3 signaling pathway was found to mediate beta-glucan-induced IL-1β secretion, but not CXCL8 or CXCL11.

Conclusions:

  • Beta-glucan stimulates rabbit KC to secrete inflammatory cytokines CXCL8, CXCL11, and IL-1β.
  • The JAK2/STAT3 pathway is specifically involved in mediating beta-glucan-induced IL-1β production.
  • This study provides insights into the mechanisms of skin immune system injury caused by beta-glucan in rabbit dermatophytosis.
Keywords:
IL-1βJAK2/STAT3keratinocytesβ-glucan

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