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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Dec 25, 2025

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Photon-limited single-pixel imaging.

Xialin Liu, Jianhong Shi, Lei Sun

|April 1, 2020

Summary

This summary is machine-generated.

This study introduces a novel photon-limited imaging technique for high-efficiency 3D image reconstruction using just one pulse per frame. This method significantly reduces light power and acquisition time compared to conventional techniques.

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Last Updated: Dec 25, 2025

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

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Published on: October 31, 2015

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

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Published on: September 5, 2019

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

Published on: February 13, 2021

Area of Science:

Photon-limited imaging
3D image reconstruction
Optical sensing

Background:

Achieving high photon-efficiency in imaging is crucial but challenging.
Existing photon-limited imaging techniques often require substantial photon counts per frame.
Single-pixel correlated imaging systems face limitations in efficiency.

Purpose of the Study:

To develop a novel photon-limited imaging technique with high photon efficiency.
To explore the consistency of photon detection probability and light intensity distribution.
To enable high-quality 3D image reconstruction using minimal photons.

Main Methods:

Proposed a new photon-limited imaging technique leveraging photon detection probability consistency.
Utilized principles of single-pixel correlated imaging.
Conducted theoretical and experimental validation.

Main Results:

Demonstrated high-quality 3D image reconstruction with 0.01 detected photons per pixel.
Achieved high photon efficiency, significantly outperforming conventional methods.
Successfully conducted long-distance field experiments (100 km and 3 km).

Conclusions:

The novel technique achieves high photon efficiency for 3D imaging.
It drastically reduces light power and acquisition time (by two orders of magnitude).
The method is feasible for practical long-distance imaging applications.