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Related Experiment Video

Updated: Dec 25, 2025

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

On-chip fluorescence microscopy with a random microlens diffuser.

Grace Kuo, Fanglin Linda Liu, Irene Grossrubatscher

|April 1, 2020

Summary

This summary is machine-generated.

We developed a compact, widefield fluorescence microscope using a diffuser instead of traditional optics. This system enables 3D imaging and refocusing from a single snapshot, even in low light.

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Area of Science:

Microscopy
Optical Engineering
Computational Imaging

Background:

Traditional microscopes are bulky and complex.
Existing widefield microscopy lacks 3D imaging capabilities from single acquisitions.
Low-light imaging presents significant challenges in microscopy.

Purpose of the Study:

To introduce a compact, on-chip widefield fluorescence microscope.
To enable 3D imaging and post-processing refocusability using a diffuser.
To improve low-light performance in microscopy.

Main Methods:

Utilized a diffuser placed near an image sensor, replacing conventional optics.
Developed a calibration scheme and physics-based forward model for image reconstruction.
Introduced a random microlens diffuser for noise robustness and enhanced low-light performance.

Main Results:

Achieved a compact and easily assembled microscope system.
Demonstrated a working distance exceeding 1.5 mm.
Enabled 3D imaging and refocusability from single-shot acquisitions.
Showcased robust performance in low-light conditions with the microlens diffuser.

Conclusions:

The diffuser-based microscope offers a compact and versatile alternative to traditional systems.
The proposed system facilitates 3D imaging and post-processing flexibility.
The random microlens diffuser design enhances performance in challenging low-light scenarios.