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Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

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The application of self-assembled nanostructures in peptide-based subunit vaccine development

Guangzu Zhao¹, Saranya Chandrudu¹, Mariusz Skwarczynski¹

¹School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane, Queensland 4072, Australia.

European Polymer Journal

|April 1, 2020

Abstract:

Peptide based-vaccines are becoming one of the most widely investigated prophylactic and therapeutic health care interventions against a variety of diseases, including cancer. However, the lack of a safe and highly efficient adjuvant (immune stimulant) is regarded as the biggest obstacle to vaccine development. The incorporation of a peptide antigen in a nanostructure-based delivery system was recently shown to overcome this obstacle. Nanostructures are often formed from antigens conjugated to molecules such as polymers, lipids, and peptide, with the help of self-assembly phenomenon. This review describes the application of self-assembly process for the production of peptide-based vaccine candidates and the ability of these nanostructures to stimulate humoral and cellular immune responses.

Keywords:

(C18)2, N,N-dioctadecyl succinamic acid APC, antigen-presenting cell BMA, butyl methacrylate C16, 2-(R/S)-hexadecanoic acid CFA, complete Freund's adjuvant Conjugation CuAAC, copper-catalyzed azide-alkyne cycloaddition DLS, dynamic light scattering ELISA, enzyme-linked immunosorbent assay FDA, Food and Drug Administration GAS, group A streptococcus HCV, hepatitis C virus HIV, human immunodeficiency virus HPV, human papilloma virus IFA, incomplete Freund’s adjuvant IgG, immunoglobulin G LCP, lipid core peptide Lipopeptide Nanofiber Nanoparticle OVA, ovalbumin PADRE, pan DR epitope PBS, phosphate-buffered saline PDSMA, pyridyl disulfide methacrylamide PEG-PPS, poly(ethylene glycol)-stabilized poly(propylene sulfide) core nanoparticle Pam2Cys, dipalmitoyl-S-glyceryl cysteine Pam3Cys, tripalmitoyl-S-glyceryl cysteine PbCSP, Plasmodium berghei circumsporozoite protein Polymer SAP, self-assembling polypeptide SARS, severe acute respiratory syndrome Self-assembly T-VEC, talimogene laherparepvec TEM, transmission electron microscopy TLR2, toll-like receptor 2 TLR4, toll-like receptor 4 TLR9, toll-like receptor 9 VLP, virus-like particle Vaccine

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