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Evaluation of Serum miRNA-24, miRNA-29a and miRNA-502-3p Expression in PCOS Subjects: Correlation with Biochemical
Dipti Nanda1, Sathiya Priya Chandrasekaran1, Vidhya Ramachandran1
1Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamil Nadu 608002 India.
Abstract:
MicroRNAs (miRNAs) are small, endogenous, non-coding, single stranded RNAs which play a role in the regulation of gene expression and function. Therefore, the analysis of differentially expressed miRNAs are of great importance in disease diagnosis. This study is focussed on the differential expression of miRNAs in serum of PCOS subjects compared to control and their correlation with metabolic and endocrine parameters. Anthropometry, hormone concentrations and biochemical characteristics were measured in healthy (n = 20) and PCOS (n = 20) subjects. MiR-24, miR-29a and miR-502-3p were determined in serum by quantitative RT-PCR. The levels of miR-24 was significantly decreased in PCOS subjects (P = 0.00) compared to control. No significant difference was observed in the levels of miR-29a and miR-502-3p in PCOS and control subjects. MiR-24 showed significant inverse correlation with BMI, glucose, insulin, FIRI, HOMA, LH, testosterone, TG, and LH:FSH ratio whereas HDL levels showed significant positive association with miR-24 and miR-29a. LH showed significant negative association with miR-29a. No correlation was observed between the expression of miR-502-3p with any of the studied parameters. The receiver operating characteristic curve for miR-24 alone showed a significant discriminative capacity. The study suggests that serum miR-24 analysis in PCOS patients could be of diagnostic value that can be used as a biomarker for PCOS.
Insights
Serum miR-24 levels are significantly decreased in women with Polycystic Ovary Syndrome (PCOS). This finding suggests that miR-24 may serve as a valuable biomarker for PCOS diagnosis.
Area of Science:
- Endocrinology
- Molecular Biology
- Genetics
Background:
- MicroRNAs (miRNAs) regulate gene expression and are crucial in disease diagnosis.
- Differential expression analysis of miRNAs in serum is important for identifying disease biomarkers.
- Polycystic Ovary Syndrome (PCOS) is a complex endocrine disorder with various metabolic and hormonal implications.
Purpose of the Study:
- To investigate the differential expression of specific miRNAs (miR-24, miR-29a, miR-502-3p) in the serum of PCOS subjects compared to healthy controls.
- To correlate the levels of these miRNAs with metabolic and endocrine parameters in PCOS patients.
Main Methods:
- Serum samples were collected from 20 PCOS subjects and 20 healthy controls.
- Quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to determine miRNA levels.
- Anthropometric, hormonal, and biochemical parameters were measured.
Main Results:
- miR-24 levels were significantly decreased in PCOS subjects compared to controls (P=0.00).
- No significant differences in miR-29a and miR-502-3p levels were observed between groups.
- miR-24 showed inverse correlations with BMI, glucose, insulin, LH, and testosterone, and a positive association with HDL.
- Receiver operating characteristic (ROC) curve analysis indicated significant discriminative capacity for miR-24.
Conclusions:
- Serum miR-24 is significantly downregulated in PCOS patients.
- miR-24 holds potential as a diagnostic biomarker for PCOS.
- Further research is warranted to explore the role of miR-29a and miR-502-3p in PCOS.
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