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Screening of long non-coding RNAs markers in plasma of children with chronic gastritis
Zhen Gu1, Hua-Qin Shen1, Pei-Hua Fu1
1Department of Pediatrics, Shanghai Pudong New District Zhoupu Hospital, Shanghai 201318, China.
Insights
This study identified specific long non-coding RNAs (lncRNAs) in children with chronic gastritis. These lncRNAs, RP11-697M17.1 and UNQ697, show potential as plasma biomarkers for early detection.
Area of Science:
- Molecular Biology
- Pediatric Gastroenterology
- Genomics
Background:
- Chronic gastritis in children is a significant health concern.
- Understanding the molecular mechanisms underlying pediatric chronic gastritis is crucial for effective diagnosis and treatment.
Purpose of the Study:
- To detect and analyze long non-coding RNA (lncRNA) expression profiles in the plasma of children with chronic gastritis.
- To explore the potential biological functions and signaling pathways involving these lncRNAs.
- To identify potential plasma biomarkers for chronic gastritis in pediatric patients.
Main Methods:
- Plasma samples were collected from pediatric patients diagnosed with chronic gastritis and healthy controls.
- Gene chip technology was employed to determine lncRNA expression profiles.
- Bioinformatic analysis, including KEGG pathway enrichment, was performed to analyze differential expression and functional implications.
Main Results:
- Five lncRNAs were significantly up-regulated, including RP11-697M17.1, and five were down-regulated, including UNQ697.
- RP11-697M17.1 and UNQ697 exhibited the highest degrees of up-regulation and down-regulation, respectively.
- KEGG pathway analysis indicated enrichment in pathways such as PI3K-Akt signaling and metabolic pathways for up-regulated and down-regulated lncRNAs, respectively.
Conclusions:
- The distinct expression profiles of lncRNAs in children with chronic gastritis were identified.
- lncRNA RP11-697M17.1 and lncRNA UNQ697 may serve as promising plasma biomarkers for predicting chronic gastritis in children.
Objective:
The study aimed to detect and analyze long non-coding RNAs (lncRNAs) in plasma of children diagnosed with chronic gastritis, and to explore its biological functions and involved signaling pathways.
Methods:
The plasma samples were collected from six children that were diagnosed with chronic gastritis by physical examination, gastroscopy, and pathological examination and six healthy children. The plasma samples were assayed for determining the expression profiles of lncRNA based upon the gen chip detection. The specific expression of lcnRNA in plasma of children with chronic gastritis was analyzed and its biological functions were speculated.
Results:
Five lncRNAs (RP11-697M17.1, RP11-388M20.9, AFAP1-AS1, BC062758, and XLOC001406) were significantly up-regulated, and five lncRNAs (UNQ697, BX571672.5, CYP4F35P, ANKRD20A5P, and AL832737) were observed to be significantly down-regulated. The lncRNAs RP11-697M17.1, and UNQ697 were detected with the highest up-regulation and down-regulation, respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that the up-regulated lncRNAs were significantly enriched in 20 signaling pathways such as phosphoinositide-3-kinase-protein kinase B (PI3K-Akt) pathway, and the down-regulated lncRNAs target genes were significantly enriched in 20 signaling pathways such as the metabolic pathway.
Conclusion:
The analysis of the lncRNA expression profiles in plasma of children with chronic gastritis revealed that the lncRNA RP11-697M17.1, and lncRNA UNQ697 may act as plasma markers for predicting chronic gastritis in children.
