Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recombinational switch for gene expression.

J Zieg, M Silverman, M Hilmen

    Science (New York, N.Y.)
    |April 8, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Determinants of premature atherosclerosis in children with end-stage renal disease.

    Physiological research·2011
    Same author

    Perception of exercise induced asthma by children and their parents.

    Archives of disease in childhood·2003
    Same author

    Lung function and low birth weight.

    Archives of disease in childhood·2003
    Same author

    An adult model of exclusive viral wheeze: inflammation in the upper and lower respiratory tracts.

    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2003
    Same author

    The economic impact of preschool asthma and wheeze.

    The European respiratory journal·2003
    Same author

    Screening for problem drinking in older people referred to a mental health service: a comparison of CAGE and AUDIT.

    Aging & mental health·2003
    Same journal

    Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

    Science (New York, N.Y.)·2026
    Same journal

    Local signals, systemic decline.

    Science (New York, N.Y.)·2026
    Same journal

    The mechanics of liver regeneration.

    Science (New York, N.Y.)·2026
    Same journal

    Computing in a memory with physics.

    Science (New York, N.Y.)·2026
    Same journal

    Retraction.

    Science (New York, N.Y.)·2026
    Same journal

    Making time.

    Science (New York, N.Y.)·2026
    See all related articles

    Salmonella flagellar gene expression is controlled by DNA inversions. Researchers found that changes in the DNA sequence adjacent to the H2 gene correlate with gene activity, suggesting inversions regulate which flagellar antigen is produced.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Flagellar antigens in Salmonella are encoded by two genes, H1 and H2.
    • Gene expression is regulated to allow only one gene to be active at a time, known as phase variation.

    Purpose of the Study:

    • To investigate the molecular mechanisms underlying flagellar gene phase variation in Salmonella.
    • To identify the genetic elements responsible for regulating H1 and H2 gene expression.

    Main Methods:

    • Molecular cloning techniques were employed to isolate DNA segments containing the H1 and H2 genetic loci.
    • Heteroduplex analysis was used to examine the structure of the cloned DNA fragments.
    • Correlation analysis was performed between gene sequence and expression state.

    Related Experiment Videos

    Main Results:

    • An anomaly, identified as an inversion, was detected in the cloned DNA fragment adjacent to the H2 gene.
    • A direct correlation was established between the phase state of the H2 gene and the sequence configuration of the adjacent inverted segment.

    Conclusions:

    • The findings suggest that DNA inversion in the region adjacent to the H2 gene is the critical event determining flagellar gene expression phase in Salmonella.
    • This inversion mechanism provides insight into the regulation of bacterial motility and antigen expression.