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Techniques to Induce and Quantify Cellular Senescence
Published on: May 1, 2017
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CO ameliorates cellular senescence and aging by modulating the miR-34a/Sirt1 pathway
Jeongmin Park1, Jin Kim1, Yingqing Chen2,3
1Department of Biological Sciences, University of Ulsan, Ulsan, Republic of Korea.
Free Radical Research
|April 2, 2020
Summary
Carbon monoxide (CO) delays cellular senescence and aging by regulating miR-34a and Sirt1. This gas reduces aging markers and extends lifespan, offering potential anti-aging benefits.
Area of Science:
- Gerontology and molecular biology
- Cellular senescence mechanisms
- Oxidative stress and aging
Background:
- Oxidative stress drives cellular senescence and aging.
- Carbon monoxide (CO), produced by hemeoxygenase-1 (HO-1), has cytoprotective roles.
- The specific impact of CO on cellular senescence and aging remains unclear.
Purpose of the Study:
- To investigate the effect of CO on cellular senescence and aging.
- To elucidate the molecular mechanisms underlying CO's influence on aging.
- To determine CO's potential as an anti-aging therapeutic agent.
Main Methods:
- Assessed CO's effect on H2O2-induced senescence in human diploid fibroblasts (WI-38 cells).
- Measured senescence-associated secretory phenotype (SASP) markers (TNF-α, IL-6, PAI-1) and antioxidant genes (HO-1, NQO1).
- Investigated the role of miR-34a and Sirt1 in CO's anti-aging effects using inhibitors and mimics, and tested CO in Caenorhabditis elegans lifespan and liver aging models.
Main Results:
- CO significantly reduced premature senescence and SASP factor expression in WI-38 cells.
- CO increased antioxidant gene expression and enhanced Sirt1 levels via miR-34a downregulation.
- Sirt1 was identified as critical for CO's anti-aging and antioxidant properties; CO extended lifespan and delayed liver aging in C. elegans.
Conclusions:
- CO effectively delays cellular senescence and aging.
- The anti-aging effects of CO are mediated by the miR-34a/Sirt1 pathway.
- CO demonstrates potential for mitigating aging processes and age-related diseases.
Keywords:
Caenorhabditis elegansSirt1carbon monoxidehaemoxygenasemiR-34aoxidative stresssenescence-associated secretory phenotypeMore Related Videos
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