Devosia ginsengisoli sp. nov., isolated from ginseng cultivation soil

A Gram-stain-negative, strictly aerobic, motile, ivory-coloured and rod-shaped bacterium (designated Gsoil 520^T) isolated from ginseng cultivation soil was characterized by using a polyphasic approach to clarify its taxonomic position. Strain Gsoil 520^T was observed to grow optimally at 30 °C and pH 7.0 on Reasoner's 2A agar medium. The results of phylogenetic analysis, based on 16S rRNA gene sequence similarities, indicated that Gsoil 520^T belongs to the genus Devosia of the family Hyphomicrobiaceae and was most closely related to Devosia epidermidihirudinis E84^T (98.0 %), Devosia yakushimensis Yak96B^T (97.7 %), Devosia neptuniae J1^T (97.7 %) and Devosia chinhatensis IPL18^T (96.8 %). The complete genome of strain Gsoil 520^T is a presumptive circular chromosome of 4 480 314 base pairs having G+C content of 63.7 mol%. A total of 4 354 genes, 4 303 CDS and 43 rRNA genes were assigned a putative function. The major isoprenoid quinone was Q-10. The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified aminolipids (AL1 and AL3). The predominant fatty acids of strain Gsoil 520^T were C_18 : 1ω7c 11-methyl, C_16 : 0 and C_18 : 1ω7c/C_18 : 1ω6c (summed feature 8) supporting the affiliation of strain Gsoil 520^T to the genus Devosia. The low values of DNA-DNA hybridization distinguished strain Gsoil 520^T from the recognized species of the genus Devosia. Thus, the novel isolate represents a novel species of the genus Devosia, for which the name Devosia ginsengisoli sp. nov. is proposed, with the type strain Gsoil 520^T (=KACC 19440^T=LMG 30329^T).