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CellTrace Violet™ inhibits equine lymphocyte proliferation.

Kamm J Lacy1, Natalie A Parlane2, Christopher B Riley1

  • 1Massey University, Tennent Drive, Palmerston North, 4474, New Zealand.

Veterinary Immunology and Immunopathology
|April 2, 2020
PubMed
Summary

CellTrace Violet™ dye inhibits equine lymphocyte proliferation, even at low concentrations. This study advises against using this dye for assessing equine lymphocyte proliferation due to its inhibitory effects.

Keywords:
CellTraceEquineFlow cytometryLymphocyteProliferation dyes

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CellTrace Violet™ is a fluorescent dye frequently used in flow cytometry to track cell proliferation.
  • Assessing equine lymphocyte proliferation is crucial for understanding immune responses in horses.

Purpose of the Study:

  • To evaluate the efficacy and impact of CellTrace Violet™ staining on equine lymphocyte proliferation.
  • To determine if CellTrace Violet™ interferes with standard proliferation assays in equine models.

Main Methods:

  • Equine lymphocytes were activated and stained with CellTrace Violet™.
  • Proliferation was assessed using flow cytometry, microscopy, and tritiated thymidine assays.
  • Cell viability was monitored in both stained and unstained lymphocyte populations.

Main Results:

  • CellTrace Violet™ was effectively incorporated into equine lymphocytes.
  • Activated equine lymphocytes showed proliferation but were inhibited when previously stained with the dye.
  • Serial dilutions of CellTrace Violet™ did not reverse the observed inhibition.
  • Lymphocyte viability remained high (>90%) in both stained and unstained groups.

Conclusions:

  • CellTrace Violet™ demonstrates an inhibitory effect on activated equine lymphocyte proliferation.
  • The dye is not recommended for assessing lymphocyte proliferation in equine studies.
  • The precise mechanism underlying CellTrace Violet™-induced inhibition in equine lymphocytes requires further investigation.