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lncRNA - Long Non-coding RNAs

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Related Experiment Video

Updated: Dec 25, 2025

Establishment of a Co-culture System of Patient-Derived Colorectal Tumor Organoids and Tumor-Infiltrating Lymphocytes (TILs)

Establishment of a Co-culture System of Patient-Derived Colorectal Tumor Organoids and Tumor-Infiltrating Lymphocytes (TILs)

Published on: June 27, 2025

Human NKp44⁺ Group 3 Innate Lymphoid Cells Associate with Tumor-Associated Tertiary Lymphoid Structures in Colorectal

Atsuyo Ikeda¹, Takayuki Ogino², Hisako Kayama^3,4,5

¹Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.

Cancer Immunology Research

|April 2, 2020

Summary

Innate lymphoid cells (ILCs) are crucial for gut health and cancer immunity. In colorectal cancer, NKp44+ ILC3s decrease in advanced tumors, impacting tertiary lymphoid structures (TLS) and potentially tumor immunity.

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Last Updated: Dec 25, 2025

Establishment of a Co-culture System of Patient-Derived Colorectal Tumor Organoids and Tumor-Infiltrating Lymphocytes (TILs)

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Published on: June 27, 2025

Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

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Published on: December 16, 2022

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

Area of Science:

Immunology
Oncology
Gastroenterology

Background:

Innate lymphoid cells (ILCs) play roles in tissue homeostasis and cancer.
The specific function of ILCs in colorectal cancer (CRC) remains unclear.
Understanding ILCs in the CRC tumor microenvironment is critical.

Purpose of the Study:

To characterize human ILCs in normal colon and CRC tissues.
To investigate the role of ILCs in the CRC tumor immune microenvironment.
To determine the relationship between ILCs, tertiary lymphoid structures (TLS), and CRC progression.

Main Methods:

Human normal colon and CRC tissues were obtained from patients.
Cells were isolated using enzymatic digestion.
NKp44+ ILC3s and their gene expression related to TLS formation were analyzed.

Main Results:

NKp44+ ILC3s expressing TLS formation genes (LTA, LTB, TNF) were found in normal mucosa and early-stage (T1/T2) CRC.
The number of NKp44+ ILC3s significantly decreased in advanced (T3/T4) CRC.
Advanced CRC showed reduced expression of TLS-related genes in NKp44+ ILC3s and stromal cells (CXCL13, CCL19, CCL21).
Decreased NKp44+ ILC3s correlated with reduced TLS density in tumors.

Conclusions:

NKp44+ ILC3s infiltrate CRC tissues but decline in advanced stages.
The reduction in NKp44+ ILC3s is associated with decreased TLS formation in T3/T4 tumors.
These findings suggest a role for NKp44+ ILC3s and TLS in CRC progression and immune response.