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Updated: Dec 25, 2025

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
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Vitamin D Suppresses Ovarian Cancer Growth and Invasion by Targeting Long Non-Coding RNA CCAT2

Liye Wang1, Shuang Zhou1, Bin Guo1

  • 1Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston, TX 77204, USA.

International Journal of Molecular Sciences
|April 2, 2020
PubMed

Insights

Vitamin D

Area of Science:

  • Oncology
  • Molecular Biology
  • Nutraceuticals

Background:

  • Ovarian cancer is a leading cause of cancer death in women globally.
  • Current treatments for ovarian cancer have limited efficacy, necessitating new biomarkers and prevention strategies.
  • Calcitriol, the active form of vitamin D, exhibits anti-cancer properties via unknown mechanisms.

Purpose of the Study:

  • To investigate the role of calcitriol in ovarian cancer.
  • To explore the effect of calcitriol on the long non-coding RNA CCAT2 (colon cancer associated transcript 2) in ovarian cancer.
  • To elucidate the anti-cancer mechanism of vitamin D in ovarian cancer.

Main Methods:

  • Ovarian cancer cell lines were treated with calcitriol.
  • CCAT2 expression levels were measured.
  • Cell proliferation, migration, and invasion assays were performed.
  • The binding of transcription factor TCF7L2 (TCF4) to the MYC promoter and c-Myc protein expression were analyzed.

Main Results:

  • Calcitriol significantly inhibited CCAT2 expression in ovarian cancer cells.
  • Calcitriol treatment reduced ovarian cancer cell proliferation, migration, and invasion.
  • Calcitriol decreased TCF7L2 binding to the MYC promoter, leading to reduced c-Myc protein expression.
  • These effects were linked to the inhibition of CCAT2 by calcitriol.

Conclusions:

  • Calcitriol demonstrates a novel anti-cancer mechanism in ovarian cancer by targeting CCAT2.
  • Vitamin D may serve as a potential nutraceutical for ovarian cancer prevention.
  • Targeting CCAT2 offers a new therapeutic strategy for ovarian cancer.
Keywords:
CCAT2cancer preventionlong non-coding RNAovarian cancervitamin D

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