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Wavelength-encoded laser particles for massively multiplexed cell tagging.

Nicola Martino1, Sheldon J J Kwok1,2, Andreas C Liapis1

  • 1Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 50 Blossom Street, Boston, MA 02114, USA.

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|April 2, 2020
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Summary

Researchers developed novel intracellular laser particles for large-scale single-cell tracking. These probes enable massive spectral multiplexing, allowing real-time imaging of thousands of cells and their behaviors in 3D models.

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Area of Science:

  • Biophysics
  • Optical Engineering
  • Cell Biology

Background:

  • Cellular heterogeneity is crucial in complex biological systems, necessitating advanced single-cell analysis techniques.
  • Current optical imaging methods face limitations in distinguishing numerous individual cells due to spectral crosstalk.

Purpose of the Study:

  • To investigate the use of intracellular laser particles for large-scale, spectrally multiplexed cell tracking.
  • To assess the stability, biocompatibility, and imaging utility of these novel probes.

Main Methods:

  • Development of silica-coated semiconductor microcavity laser particles with single-mode emission (1170-1580 nm).
  • In vitro evaluation of probe stability and biocompatibility.
  • Demonstration of wavelength-multiplexed cell tagging and real-time imaging in a 3D tumor model.

Main Results:

  • Laser particles exhibit single-mode emission with sub-nm linewidths, enabling massive spectral multiplexing.
  • Probes demonstrated stability and biocompatibility in vitro.
  • Real-time tracking of thousands of individual cells over several days was achieved, revealing diverse behavioral phenotypes.

Conclusions:

  • Intracellular laser particles offer a powerful new tool for large-scale, high-resolution single-cell analysis.
  • This technology overcomes limitations of conventional fluorescence methods for tracking cellular heterogeneity.