Lysinibacillus cavernae sp. nov., isolated from cave soil.
Yu Kan1, Xue-Ke Niu1, Manik Prabhu Narsing Rao2,3
1Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou and Guizhou Talent Base for Microbiology and Human Health, Key Laboratory of Medical Microbiology and Parasitology of Education, Department of Guizhou, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, People's Republic of China.
Archives of Microbiology
|April 2, 2020
Summary
A novel bacterium, Lysinibacillus cavernae sp. nov. SYSU K30005T, was discovered in a Chinese karst cave soil sample. This finding expands the known diversity within the Lysinibacillus genus.
Area of Science:
- Microbiology
- Bacteriology
- Environmental Science
Background:
- Soil microbial communities harbor diverse bacterial species.
- Karst cave environments present unique ecological niches for microbial life.
- The genus Lysinibacillus comprises spore-forming bacteria with various environmental roles.
Purpose of the Study:
- To isolate and characterize a novel bacterium from a karst cave soil sample.
- To determine the taxonomic affiliation of the isolated strain using polyphasic characterization.
- To propose a new species within the Lysinibacillus genus.
Main Methods:
- Isolation and cultivation of bacteria from soil.
- Gram staining, motility tests, and endospore observation.
- 16S rRNA gene sequencing and phylogenetic analysis.
- Physiological and biochemical characterization (temperature, pH, NaCl tolerance).
- Cell wall composition, fatty acid analysis, and polar lipid profiling.
- Genomic DNA G+C content determination.
- Average nucleotide identity (ANI) calculations.
Main Results:
- A Gram-positive, motile, rod-shaped bacterium (SYSU K30005T) was isolated.
- Phylogenetic analysis based on 16S rRNA gene sequences indicated affiliation with the Lysinibacillus genus.
- Optimal growth conditions: 28°C, pH 7.0, 3.5% NaCl.
- Unique cell wall peptidoglycan type (A4α), cell wall sugars (ribose, galactose, mannose), predominant menaquinone (MK-7), and fatty acid profile (iso-C15:0, anteiso-C15:0, iso-C16:0, iso-C17:0).
- Genomic DNA G+C content was 37.2 mol%.
- ANI values were below the threshold for species delineation, supporting novelty.
Conclusions:
- Strain SYSU K30005T represents a novel species of the genus Lysinibacillus.
- The proposed name for the novel species is Lysinibacillus cavernae sp. nov.
- The type strain is SYSU K30005T (KCTC 43130T = CGMCC 1.17492T).
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