Related Experiment Video
Updated: Dec 25, 2025
Isolation and Characterization of Single Cells from Zebrafish Embryos
Published on: March 12, 2016
Development and characterization of a new DRCF cell line from Indian wild strain zebrafish Danio rerio (Hamilton
Lakan Lal Meena1, Mukunda Goswami2, Aparna Chaudhari1
1Fish Genetics & Biotechnology Division, ICAR - Central Institute of Fisheries Education, Panch Marg, Off. Yari Road, Mumbai, 400061, India.
Abstract:
Danio rerio, zebrafish, has been widely used as a non-mammalian vertebrate model organism in various studies. The present research describes to develop and characterize a new cell line from a wild strain Indian zebrafish native to Brahmaputra River, Assam, India. The new cell line designated as DRCF was developed from the caudal fin of D. rerio. The cell line was successfully subcultured up to 31 passages. Growth studies revealed that cell growth of DRCF was optimal at 28 °C in L-15 medium supplemented with 20% FBS. Molecular characterization of the DRCF cell line using mitochondrial genes namely cytochrome oxidase subunit I gene (COI) and 16S rRNA authenticated the true origin of the cell line. The chromosome analysis of the DRCF cell line expressed its 50 diploid chromosome number of D. rerio. The immunocytochemical characterization of the cell line exhibited its fibroblastic morphology. The expression of the green fluorescent protein (GFP) following transfection revealed the suitability of the cell line for transfection studies.
