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Reactive Polymer Targeting dsRNA as Universal Virus Detection Platform with Enhanced Sensitivity
Jayoung Ku1,2, Sura Kim1, Jaemin Park1
1Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141 South Korea.
Biomacromolecules
|April 3, 2020
Summary
A new diagnostic platform uses reactive poly(pentafluorophenyl acrylate) (PPFPA) surfaces to detect viral infections by identifying double-stranded RNA (dsRNA). This sensitive method enables broad-spectrum virus detection without needing genomic sequences.
Area of Science:
- Biomolecular Engineering
- Nanotechnology
- Infectious Disease Diagnostics
Background:
- Reactive poly(pentafluorophenyl acrylate) (PPFPA)-grafted surfaces are effective for immobilizing biomolecules.
- Double-stranded RNA (dsRNA) is a common byproduct of viral transcription and replication.
Purpose of the Study:
- To develop a universal virus detection platform with enhanced sensitivity using PPFPA-grafted surfaces and a dsRNA-recognizing antibody.
- To create a diagnostic tool capable of detecting various viruses without prior genomic sequence knowledge.
Main Methods:
- PPFPA was grafted onto silicon substrates, with surface hydrophilicity modulated using poly(ethylene glycol).
- The J2 antibody, which recognizes dsRNA, was immobilized onto the PPFPA surface.
- A two-step detection method involving multiple fluorophore-tagged J2 antibodies was employed to enhance sensitivity.
Main Results:
- The developed surface successfully distinguished long dsRNAs from single-stranded RNAs and short dsRNAs.
- The platform differentiated foreign viral dsRNAs from cellular dsRNAs and other biomolecules in cell lysates.
- Hepatitis A and C viruses were successfully detected in infected cells using this single platform.
Conclusions:
- The PPFPA-grafted surface immobilized with the J2 antibody serves as a sensitive and universal platform for virus detection.
- This technology has the potential to be a primary diagnostic tool for determining the infection status of a wide range of viral diseases.
