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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Dec 25, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Functionally heterogeneous human satellite cells identified by single cell RNA sequencing.

Emilie Barruet¹, Steven M Garcia¹, Katharine Striedinger¹

¹Departments of Surgery and Orofacial Sciences, Division of Plastic and Reconstructive Surgery, Program in Craniofacial Biology, Eli and Edythe Broad Center of Regeneration Medicine, University of California, San Francisco, San Francisco, United States.

|April 3, 2020

Summary

This summary is machine-generated.

Researchers identified distinct human muscle stem cell (Hu-MuSC) subpopulations using single-cell RNA sequencing. A novel CAV1+ subpopulation shows enhanced engraftment and resistance to activation, revealing crucial heterogeneity in muscle regeneration.

Keywords:

Human satellite cell transcriptome human human biology medicine muscle stem cell regenerative medicine satellite cell transplantation stem cells

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Published on: October 25, 2018

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Published on: January 19, 2017

Area of Science:

Muscle stem cell biology
Cellular heterogeneity
Regenerative medicine

Background:

Murine satellite cell heterogeneity is known, but distinct human satellite cell subpopulations and their functions remain poorly understood.
Human muscle stem cells (Hu-MuSCs) are crucial for muscle repair and regeneration.
Characterizing Hu-MuSC heterogeneity is essential for advancing regenerative therapies.

Purpose of the Study:

To identify and characterize distinct subpopulations within the normal human PAX7+ satellite cell pool.
To investigate the functional relevance of identified Hu-MuSC subpopulations.
To discover novel surface markers for isolating specific Hu-MuSC subpopulations.

Main Methods:

Single-cell RNA sequencing (scRNA-seq) to analyze transcriptional profiles of individual Hu-MuSCs.
Flow cytometry for cell surface marker analysis and physical separation of subpopulations.
In vitro cell culture assays to assess activation resistance.
In vivo transplantation studies to evaluate engraftment capacity.

Main Results:

Identification of transcriptionally distinct clusters within the normal Hu-MuSC pool, consistent across individuals.
Discovery of a novel Hu-MuSC subpopulation marked by CXCR4/CD29/CD56/CAV1 (CAV1+).
CAV1+ Hu-MuSCs exhibit distinct morphology, resistance to in vitro activation, and superior in vivo engraftment compared to CAV1- cells.

Conclusions:

The normal human satellite cell pool is more heterogeneous than previously recognized.
The CAV1+ subpopulation represents a distinct, functionally specialized group of human muscle stem cells.
These findings enable the isolation of functionally distinct Hu-MuSC subpopulations for research and therapeutic applications.