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Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

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PIDS: A User-Friendly Plant DNA Fingerprint Database Management System.

Bin Jiang¹, Yikun Zhao¹, Hongmei Yi¹

¹Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.

|April 3, 2020

Summary

A new Plant International DNA-Fingerprinting System (PIDS) efficiently manages massive microsatellite DNA fingerprint data for plant breeding. This system aids in sample tracking, data analysis, and genetic assessments, supporting crop improvement and quality control.

Keywords:

DNA fingerprint algorithms database genotyping microsatellites

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Area of Science:

Plant science
Bioinformatics
Genetics

Background:

DNA fingerprinting is crucial for plant breeding, variety management, and intellectual property protection.
Managing large, heterogeneous DNA fingerprint data for storage, analysis, and retrieval is resource-intensive.
Existing systems lack efficient data processing and quality control for large-scale plant molecular research.

Purpose of the Study:

To develop an efficient database management system for handling massive microsatellite DNA fingerprint data.
To provide a tool for automatic data collection, storage, and analysis in plant molecular research.
To support plant breeders with advanced data management and genetic analysis capabilities.

Main Methods:

Development of the Plant International DNA-Fingerprinting System (PIDS) using open-source web server and free software.
Implementation of merging and comparison algorithms for efficient data handling.
Integration of capillary electrophoresis image matching for fingerprint data upload and validation.

Main Results:

PIDS offers automatic data collection, storage, and efficient management of microsatellite DNA fingerprint data.
The system facilitates genetic analyses and allows for customization of back-end functions.
PIDS successfully handles massive datasets, improving laboratory data processing and quality control.

Conclusions:

The Plant International DNA-Fingerprinting System (PIDS) is a valuable tool for plant breeders, enhancing efficiency in data management and analysis.
PIDS supports crop breeding, variety control, and protection of variety rights.
The system has potential applications in forensic science, virus, and microorganism research.