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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Related Experiment Video

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Quantitative Immunofluorescence to Measure Global Localized Translation
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Guidance for quantitative confocal microscopy

James Jonkman1, Claire M Brown2, Graham D Wright3

  • 1Advanced Optical Microscopy Facility (AOMF), University Health Network, Toronto, Ontario, Canada. james@aomf.ca.

Nature Protocols
|April 3, 2020
PubMed
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No abstract available in PubMed .

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