Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.2K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.2K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.8K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.8K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

1.3K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.3K
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

1.8K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
1.8K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.7K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.7K
X-ray Imaging01:24

X-ray Imaging

9.6K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
9.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent advances in single crystal COFs from synthesis to applications.

Chemical communications (Cambridge, England)·2025
Same author

Dielectric Constant of a Single MoO<sub>3</sub> Nanostructure.

Nano letters·2025
Same author

Photochemical and Patternable Synthesis of 2D Covalent Organic Framework Thin Film Using Dynamic Liquid/Solid Interface.

Small methods·2024
Same author

Critical Role of Surface Termination of Sapphire Substrates in Crystallographic Epitaxial Growth of MoS<sub>2</sub> Using Inorganic Molecular Precursors.

ACS nano·2023
Same author

Connection between the time distribution and length scale of dynamic heterogeneity explored by probe reorientations of different sizes.

The Journal of chemical physics·2022
Same author

Optical scanning holography for a three-dimensional equal scale display.

Optics express·2022
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Dec 25, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

10.6K

Coaxial scanning holography.

Taegeun Kim, Taewoong Kim

    Optics Letters
    |April 3, 2020
    PubMed
    Summary
    This summary is machine-generated.

    Coaxial scanning holography (CSH) simplifies optical holography by using a single beam path and geometrical phase shift for high stability and low complexity. This novel approach enables stable hologram acquisition and reconstruction with reduced apparatus requirements.

    More Related Videos

    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
    10:09

    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

    Published on: September 16, 2022

    3.1K
    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    8.7K

    Related Experiment Videos

    Last Updated: Dec 25, 2025

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    10.6K
    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
    10:09

    Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

    Published on: September 16, 2022

    3.1K
    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    8.7K

    Area of Science:

    • Optics and Photonics
    • Holography

    Background:

    • Traditional optical scanning holography relies on complex interference optics, demanding high stability and coherent light sources.
    • The intricate setup of conventional methods often leads to challenges in achieving both accuracy and simplicity.

    Purpose of the Study:

    • To introduce a novel coaxial scanning holography (CSH) technique.
    • To demonstrate a method for achieving high stability and low complexity in scanning holography.

    Main Methods:

    • Proposing a coaxial scanning holography (CSH) system.
    • Utilizing a geometrical phase shift to form a scanning beam within a single optical path.
    • Experimental optical acquisition and reconstruction of holograms using the CSH system.

    Main Results:

    • Successfully implemented coaxial scanning holography (CSH).
    • Demonstrated high stability and low complexity compared to traditional methods.
    • Verified the capability of CSH for hologram acquisition and reconstruction.

    Conclusions:

    • CSH offers a simplified and highly stable alternative for scanning holography.
    • The proposed method reduces the complexity of the optical apparatus.
    • Experimental results confirm the effectiveness of CSH for holographic applications.