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Transcriptome analysis of tongue cancer based on high‑throughput sequencing.
Mingming Tang1, Wencheng Dai1, Hao Wu2
1Department of Head and Neck Surgery, Nantong Tumor Hospital, Nantong, Jiangsu 226361, P.R. China.
Oncology Reports
|April 3, 2020
Summary
This study reveals key molecular pathways driving tongue cancer. Extracellular matrix interactions and focal adhesion signaling are crucial for tumor growth, offering potential diagnostic and therapeutic targets.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Tongue cancer's molecular basis and progression mechanisms are not fully understood.
- Identifying novel diagnostic and therapeutic targets is critical for improving patient outcomes.
Purpose of the Study:
- To elucidate the pathogenesis of tongue cancer.
- To identify potential diagnostic and therapeutic targets through molecular analysis.
Main Methods:
- RNA sequencing (RNA-Seq) was performed on four matched pairs of tongue cancer and paracancerous tissues.
- Bioinformatic analyses, including Gene Ontology and Kyoto Encyclopedia of Genes and Genomes, were used to analyze differentially expressed genes.
- Reverse transcription-quantitative PCR (RT-qPCR) validated key findings.
Main Results:
- 1,700 genes were upregulated and 2,249 genes were downregulated in tongue cancer tissues.
- Enrichment analysis highlighted pathways such as extracellular matrix (ECM) organization, cell adhesion, focal adhesion, ECM-receptor interaction, and phosphoinositide 3-kinase (PI3K)-Akt signaling.
- Upregulation of cell cycle genes and downregulation of cell adhesion/differentiation genes were observed.
Conclusions:
- The ECM-receptor interaction, focal adhesion kinase (FAK), and PI3K-Akt signaling pathways are interconnected and play a synergistic role in tongue cancer development and progression.
- These pathways represent promising targets for future diagnostic and therapeutic strategies in tongue cancer.
