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Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
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iRhom2 in the pathogenesis of oral squamous cell carcinoma

Matthew E Agwae1, Richard J Shaw1,2, Asterios Triantafyllou3

  • 1Cancer Research Centre, Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool, 200 London Road, Liverpool, L3 9TA, UK.

Molecular Biology Reports
|April 3, 2020
PubMed

Insights

iRhom2 protein is elevated in oral squamous cell carcinoma (OSCC), correlating with increased cell migration and poorer patient survival. This suggests iRhom2 plays a role in OSCC development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • iRhom2 is an inactive rhomboid protease implicated in various signaling pathways and cancer pathogenesis.
  • While iRhom1 is linked to head and neck cancer, iRhom2's role in this specific cancer type remains unexplored.

Purpose of the Study:

  • To investigate the role of iRhom2 in the development of oral squamous cell carcinoma (OSCC).
  • To determine the correlation between iRhom2 expression, clinicopathological features, and patient survival in OSCC.

Main Methods:

  • Immunoblotting was used to quantify iRhom2 protein levels in 54 OSCC tissues and 24 paired normal tissues.
  • OSCC cell lines with stable iRhom2 overexpression and knock-down were utilized to assess cell migration and proliferation.
  • Correlation analysis was performed between iRhom2 expression and patient survival and clinicopathological variables.

Main Results:

  • iRhom2 protein levels were significantly higher in OSCC tumors compared to normal tissues (P < 0.05).
  • Overexpression of iRhom2 correlated strongly with decreased patient survival (P < 0.0005) but not with other clinicopathological variables.
  • iRhom2 overexpression enhanced cell migration in OSCC cell lines without affecting proliferation, an effect reversed by iRhom2 knock-down.

Conclusions:

  • iRhom2 protein expression is upregulated in a subset of OSCC.
  • Increased iRhom2 is associated with enhanced cancer cell migration and reduced patient survival in OSCC.
  • These findings suggest iRhom2-mediated signaling is involved in OSCC pathogenesis and may represent a therapeutic target.
Keywords:
Cell migrationHead and neck squamous cell carcinomaOral squamous cell carcinomaiRhom2

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