Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.3K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tuning the interlocking in partially saturated copper(I) rotaxane complexes for O<sub>2</sub>-to-H<sub>2</sub>O electrocatalytic reduction.

Chemical communications (Cambridge, England)·2026
Same author

Role of Molecular Topology Elucidated in Unified Gels.

Journal of the American Chemical Society·2026
Same author

Protocol to identify covalent inhibitors targeting RhoA Cys16.

STAR protocols·2026
Same author

Frontiers of redox biology.

Nature chemical biology·2026
Same author

Molecular dynamics simulations elucidate the role of the F-F' loop in substrate entry into CYP3A4.

Communications chemistry·2026
Same author

Conformation-driven reversibility control in ring-opening metathesis polymerization of non-bicyclic cyclooctenes.

Nature communications·2025

Related Experiment Video

Updated: Dec 25, 2025

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
09:33

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

7.8K

Fluorescein-Containing Superoxide Probes with a Modular Copper-Based Trigger.

Fung Kit Tang1, Zuo Hang Yu1, Thomas Hin-Fung Wong1

  • 1Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China.

Chempluschem
|April 3, 2020
PubMed
Summary
This summary is machine-generated.

New fluorescein-derived probes detect superoxide (O2•−) in live cells. These probes offer high sensitivity and selectivity, enabling imaging of reactive oxygen species in cellular models.

Keywords:
copperfluorescenceoxidationreactive oxygen speciessuperoxide

More Related Videos

Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo
12:06

Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo

Published on: November 5, 2013

15.0K
Quantification of Reactive Oxygen Species Using 2&#8242;,7&#8242;-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in M&#252;ller Glial Cells
14:25

Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells

Published on: May 13, 2022

7.4K

Related Experiment Videos

Last Updated: Dec 25, 2025

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
09:33

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

7.8K
Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo
12:06

Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo

Published on: November 5, 2013

15.0K
Quantification of Reactive Oxygen Species Using 2&#8242;,7&#8242;-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in M&#252;ller Glial Cells
14:25

Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells

Published on: May 13, 2022

7.4K

Area of Science:

  • Chemical Biology
  • Biomedical Imaging
  • Reactive Oxygen Species Detection

Background:

  • Superoxide (O2•−) is a key reactive oxygen species involved in cellular signaling and disease.
  • Existing superoxide probes often lack sensitivity, selectivity, or require complex activation mechanisms.
  • Developing robust and versatile probes for intracellular superoxide detection remains a critical challenge.

Purpose of the Study:

  • To develop novel fluorescein-derived probes for sensitive and selective imaging of superoxide in live cells.
  • To investigate the structure-activity relationship of copper(II) complexes for superoxide sensing.
  • To demonstrate the utility of these probes in monitoring dynamic changes of superoxide levels in cellular models.

Main Methods:

  • Design and synthesis of fluorescein-derived probes incorporating a copper(II) complex.
  • Activation mechanism involving superoxide-initiated ether bond cleavage and uncaging of a fluorescein reporter.
  • Characterization of probe performance, including sensitivity, selectivity, and fluorescence enhancement.
  • Structure-activity relationship studies of the copper(II) superoxide trigger.
  • Live-cell imaging experiments in HeLa, HEK293T, and RAW 264.7 cells.

Main Results:

  • Developed sensitive ( >30-fold fluorescence enhancement) and highly selective superoxide probes.
  • Demonstrated modularity of the bond cleavage strategy for adapting different fluorescent reporters.
  • Identified the importance of the copper(II) secondary coordination environment for probe performance.
  • Successfully imaged intracellular superoxide level changes in response to menadione stimulation.
  • Applied probes to visualize superoxide dynamics in a cellular inflammation model.

Conclusions:

  • Fluorescein-derived copper(II) probes provide a powerful platform for sensitive and selective superoxide detection in live cells.
  • The modular design allows for versatile probe development for various biological applications.
  • These probes are valuable tools for studying the role of superoxide in cellular processes and disease models.