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Physiological characterization of strain DCB-1, a unique dehalogenating sulfidogenic bacterium
T O Stevens1, T G Linkfield, J M Tiedje
1Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1325.
Applied and Environmental Microbiology
|December 1, 1988
Summary
Strain DCB-1, a bacterium performing reductive dehalogenation, grows on pyruvate. Thiosulfate and sulfite stimulate its growth, indicating it
Area of Science:
- Microbiology
- Environmental Science
- Biochemistry
Background:
- Strain DCB-1 is an obligate anaerobe known for reductive dehalogenation of halobenzoates.
- Previous studies indicated growth solely on pyruvate supplemented with ruminal fluid.
Purpose of the Study:
- To investigate the growth-stimulating effects of various electron acceptors on strain DCB-1.
- To characterize the metabolic pathways and byproducts of strain DCB-1 under different conditions.
Main Methods:
- Culturing strain DCB-1 with various electron acceptors in mineral medium.
- Analyzing carbon source utilization and metabolic products using analytical techniques.
- Detecting specific bacterial components like cytochrome c and desulfoviridin.
Main Results:
- Thiosulfate and sulfite significantly stimulated growth, with sulfide production observed from thiosulfate.
- Pyruvate served as the sole carbon source in mineral medium; other substrates required ruminal fluid and thiosulfate.
- Metabolic analysis revealed pyruvate conversion to acetate and lactate, with transient accumulation of succinate and fumarate.
- Hydrogen addition to pyruvate-grown cultures altered carbon flow, decreasing cell yield.
Conclusions:
- Strain DCB-1 exhibits enhanced growth with thiosulfate and sulfite, suggesting a role for these compounds in its metabolism.
- The bacterium demonstrates a unique metabolic profile, converting pyruvate to various organic acids.
- Strain DCB-1 represents a novel type of sulfidogenic bacterium, contributing to our understanding of anaerobic microbial communities.