High-Quality Genome Assembly of Peronospora destructor, the Causal Agent of Onion Downy Mildew
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Updated: Dec 25, 2025

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
Published on: March 31, 2017
Karthi Natesan1, Ji Yeon Park1, Cheol-Woo Kim2
1National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of Korea.
Abstract:
Peronospora destructor is an obligate biotrophic oomycete that causes downy mildew on onion (Allium cepa). Onion is an important crop worldwide, but its production is affected by this pathogen. We sequenced the genome of P. destructor using the PacBio sequencing platform, and de novo assembly resulted in 74 contigs with a total contig size of 29.3 Mb and 48.48% GC content. Here, we report the first high-quality genome sequence of P. destructor and its comparison with the genome assemblies of other oomycetes. The genome is a very useful resource to serve as a reference for analysis of P. destructor isolates and for comparative genomic studies of the biotrophic oomycetes.
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