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Rat hepatic microsomal cytochrome b5. A simple large-scale purification procedure and antibody production by
J Carlsen1, K Christiansen, H M Jensen
1Department of Biochemistry C, Panum Institute, University of Copenhagen, Denmark.
The Biochemical Journal
|December 15, 1988
Abstract:
Cytochrome b5 from rat liver microsomes (microsomal fractions) was purified in its native form. The procedure described has great capacity, is fast, and the final product is pure as judged from SDS/polyacrylamide-gel electrophoresis. Antibodies to cytochrome b5 are obtained after administration of the antigen inserted into small unilamellar lipid vesicles.