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Thiol-disulfide homeostasis in children with celiac disease

Atakan Comba1, Ayşe Semra Güreser2, Djursun Karasartova2

  • 1Department of Pediatrics, Faculty of Medicine, Training and Research Hospital, Hitit University, Çorum, Turkey.

Pediatrics International : Official Journal of the Japan Pediatric Society
|April 3, 2020
PubMed

Insights

Celiac disease disrupts thiol-disulfide balance, a key antioxidant defense in children. A gluten-free diet can help restore this balance, improving gastrointestinal health.

Area of Science:

  • Biochemistry
  • Pediatric Gastroenterology
  • Oxidative Stress Research

Background:

  • Celiac disease involves enterocyte damage from toxic gliadin peptides, leading to oxidative stress.
  • Thiols are crucial antioxidants that protect against oxidative damage.
  • This study investigates thiol-disulfide homeostasis alterations in pediatric celiac disease.

Purpose of the Study:

  • To examine changes in thiol-disulfide homeostasis in children diagnosed with celiac disease.
  • To compare these changes with those in children with functional gastrointestinal disorders and healthy controls.
  • To assess the impact of diet on thiol-disulfide balance in celiac disease patients.

Main Methods:

  • Serum samples were collected from children with celiac disease, functional gastrointestinal disorders, and healthy controls.
  • Measurements included native thiol, total thiol, and disulfide levels.
  • Calculated ratios such as disulfide/total thiol and native thiol/total thiol were analyzed.

Main Results:

  • Children with celiac disease exhibited significantly lower total thiol and disulfide levels compared to healthy children.
  • Patients with celiac disease on a gluten-containing diet showed lower native and total thiol levels than those on a gluten-free diet.
  • The study included 172 children (52.3% girls), with a mean age of 8.6 years.

Conclusions:

  • Thiol-disulfide homeostasis, vital for gastrointestinal antioxidant defense, is impaired in pediatric celiac disease.
  • Adherence to a gluten-free diet partially reverses the observed decline in thiol-disulfide balance.
  • These findings highlight the role of oxidative stress and the benefits of dietary intervention in celiac disease.
Abstract

Keywords:
celiac diseasechilddisulfideoxidative stressthiol

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