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Tantalum Oxide Nanoparticle-Based Mass Tag for Mass Cytometry.

Yefeng Zhang1, Nick Zabinyakov2, Daniel Majonis2

  • 1Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada.

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Researchers developed novel tantalum oxide nanoparticle (NP)-based mass tags for mass cytometry (MC). This advancement enables higher dimensional single-cell analysis, expanding the potential of MC immunoassays.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Immunology

Background:

  • Mass cytometry (MC) is a high-dimensional single-cell analysis technique using metal-tagged antibodies.
  • Current MC methods can measure over 40 parameters, with potential for up to 135 parameters.
  • Development of new elemental mass tags is crucial for expanding MC capabilities.

Purpose of the Study:

  • To develop and characterize tantalum oxide nanoparticle (NP)-based mass tags for MC immunoassays.
  • To demonstrate the utility of these novel NP-based mass tags in high-dimensional single-cell analysis.
  • To integrate these new reagents into existing MC workflows.

Main Methods:

  • Synthesized uniform-sized amine-functionalized tantalum oxide NPs using a reverse microemulsion method.
  • Modified NPs with azide groups and conjugated them to dibenzocyclooctyne (DBCO)-functionalized antibodies (Abs).
  • Fabricated and tested three specific Ab-NP conjugates (TaO2-PEG2-goat antimouse, TaO2-PEG2-CD25, TaO2-PEG2-CD196) in MC immunoassays.

Main Results:

  • Successfully synthesized and functionalized tantalum oxide NPs for antibody conjugation.
  • Demonstrated effective detection of CD20 biomarkers on Ramos cells using a TaO2-PEG2-goat antimouse conjugate.
  • Showcased successful integration of TaO2-PEG2-CD25 and TaO2-PEG2-CD196 conjugates into commercial panels for immune profiling.

Conclusions:

  • Tantalum oxide NPs represent a viable new mass tag for MC immunoassays.
  • This NP-based approach enhances the multiplexing capacity of MC.
  • The developed conjugates facilitate high-dimensional single-cell immune profiling.