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Nuclear DNA content in prostatic carcinoma measured by flow cytometry: a retrospective study on paraffin-embedded
H Willumsen1, J Thorup, T Nørgaard
1Dept. of Surgery A, Central Hospital, Hillerød, Denmark.
APMIS. Supplementum
|January 1, 1988
Abstract:
The nuclear DNA content in 67 prostatic carcinomas was measured by flow cytometry of nuclear suspensions prepared from paraffin-embedded tissue blocks. A method was developed that provided nuclear suspensions of good quality whether tissue blocks from transurethral resections or transvesical prostatectomies were used. Thirty (43.7%) of the tumors were found to be aneuploid. No significant correlation between clinical stage or histological grade and aneuploidy was demonstrated.