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Connective tissue growth factor mediates mouse spermatogonial migration associated with differentiation
Ziyi Chen1, Xiaofeng Li1, Jing Jin1
1School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong Special Administrative Region.
Biochimica Et Biophysica Acta. Molecular Cell Research
|April 3, 2020
Summary
Connective tissue growth factor (CTGF) promotes spermatogonial migration during differentiation. This study reveals CTGF
Area of Science:
- Reproductive Biology
- Developmental Biology
- Cell Biology
Background:
- Spermatogonia migration is crucial for testicular development and function.
- Mechanisms regulating spermatogonial migration during differentiation are largely unknown.
Purpose of the Study:
- To investigate the role of connective tissue growth factor (CTGF) in regulating spermatogonial migration associated with differentiation.
- To elucidate the molecular mechanisms underlying CTGF-mediated spermatogonial migration.
Main Methods:
- Induction of spermatogonial differentiation using retinoic acid (RA).
- Analysis of CTGF expression and its role in spermatogonial migration using recombinant CTGF and gene silencing techniques.
- Investigation of CTGF interaction with β1-integrin.
Main Results:
- Retinoic acid-induced differentiation increased spermatogonial migration and CTGF expression.
- Recombinant CTGF promoted migration, while CTGF silencing suppressed it.
- CTGF demonstrated both paracrine and autocrine roles in migration and interacted with β1-integrin.
Conclusions:
- CTGF plays a significant role in regulating spermatogonial migration during differentiation.
- CTGF's interaction with β1-integrin is a key mechanism in this process.
- Findings offer potential insights for diagnosing and treating male infertility.
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