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Sudan black B as a histological stain for polymeric biomaterials embedded in glycol methacrylate
E A Hoeksma1, B van der Lei, M F Jonkman
1Centre for Medical Electron Microscopy, University of Groningen, The Netherlands.
Abstract:
Sudan black B, usually a stain for all kinds of lipid, turned out to be an excellent histological stain for polymeric biomaterials embedded in glycol methacrylate. Staining the surrounding connective tissue with toluidine blue-basic fuchsin makes details of the polymer-tissue interface clearly visible. Sudan black B might be used to visualize the biodegradation process of polymeric biomaterials.
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