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Related Concept Videos

Proteomics01:33

Proteomics

9.1K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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The Proteasome02:18

The Proteasome

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The Proteasome01:13

The Proteasome

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Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
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The Proteasome02:18

The Proteasome

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Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
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Updated: Dec 25, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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What is proteomics?

Andrew James McArdle1, Stephanie Menikou2

  • 1Department of Infectious Disease, Imperial College London, London, UK andrew.mcardle@gmail.com.

Archives of Disease in Childhood. Education and Practice Edition
|April 4, 2020
PubMed
Summary
This summary is machine-generated.

Proteomics, the study of proteins, offers direct insights into biological systems for pediatricians. This approach complements other

Keywords:
paediatricsproteomics

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Area of Science:

  • * Biochemistry and Molecular Biology
  • * Clinical Research

Background:

  • * Proteomics involves the large-scale study of proteins within biological systems.
  • * Understanding protein expression is crucial for deciphering complex biological functions.

Purpose of the Study:

  • * To introduce the field of proteomics to pediatricians.
  • * To present current applications of proteomics in addressing pediatric health challenges.
  • * To highlight the advantages and challenges of proteomics in clinical research.

Main Methods:

  • * Description of various proteomic approaches, with a focus on tandem mass spectrometry ('bottom-up proteomics').
  • * Explanation of the experimental and computational methodologies involved.
  • * Comparison with transcriptomics, noting proteomics provides direct protein information.

Main Results:

  • * Proteomics offers direct protein data, contrasting with RNA-based transcriptomics.
  • * Challenges include lower data depth and confident mass spectra identification.
  • * Proteomics effectively complements other 'omics' approaches.

Conclusions:

  • * Proteomics holds significant promise for advancing pediatric research and clinical questions.
  • * Effective utilization of proteomics can lead to novel diagnostic and therapeutic strategies in pediatrics.
  • * The integration of proteomics with other 'omics' enhances biological understanding.